1988 Volume 27 Issue 4 Pages 477-483
A patient of ovarian cancer with hyperamylasemia was treated by chemotherapy and surgical specimens were obtained after the labelling in vivo with bromodeoxyuridine (BrdU).
Amylase was detected in the cytoplasm and BrdU was localized in the nuclei of neoplastic cells by immunoperoxidase method (IPX), and amylase positive cells showed no positive image of BrdU.
Labelling index, determined by IPX with anti-BrdU monoclonal antibody, revealed 11.6% and less than 1% before and after chemotherapy, respectively.
Since amylase was considered to express the characteristics of cytoplasm and BrdU showed the function of nuclei, it was seemed amylase and BrdU would be regard as independent parameters in the ovarian cancer. Immunohistochemical double staining, adopted on stamp smear, clarified the relation between the localization of amylase and that of BrdU at a time. Immunohistochemical double staining was regarded as a useful technique on histological and cytological reseaches.
