Abstract
Staphylococcus aureus was applied to wounds on the backs of mice to prepare models of wound infections for examining changes in the wounds over time. Every 3 hours for up to 24 hours after the application of bacteria, and then at 48 and 120 hours, seven mice were sacrificed. Five were frozen and stored, followed by the quantification and culture of the wounded areas. The wounded areas of 2 mice were fixed in 5% formalin for observation. At 6 hours after the application of bacteria, no inflammatory cells infiltrated the wounded areas. At 9 hours, the bacteria proliferated around the wound, and many neutrophils that engulfed the bacteria infiltrated. At 12-21 hours, the number of bacteria on the surface layer increased. At 21-120 hours, the number of bacteria decreased. These relationships were significant. Our models revealed the course of wound and puncture infections, providing a potentially useful tool for investigating their prevention and treatment.
