Abstract
Immunohistochemical approach in pain research is one of the effective methods to search for existence of some substance and investigate localization of receptors or neurotransmitters. Although immunohistochemical staining is widely used, the method sometimes brings confusion to those who are unfamiliar to it. Here we give comments on preparations of tissues and general procedures of immunohistochemical methods for beginner of pain research. Taking streptavidin-biotinylated peroxidase complex method as a case of enzyme labeled immunohistochemical stainings, we refer to practical arrangements of these procedures and important factors to be paid attentions to when the methods do not work well.
