Adsorption Properties of Bovine Serum Albumin on to Electrically Polarized Hydroxyapatite Powder

Abstract

Electrically polarized hydroxyapatite (HA) ceramics have been proved to promote the formation of bone mineral like apatite in vitro and new bone in vivo. To clarify the adsorption properties of the electrically polarized HA powder, column chromatography of the polarized HA powder was carried out. The adsorption tests for bovine serum albumin protein was performed in 0.01 M phosphate buffer solution adjusted to pH 6.60 and 7.40. The adsorption ability of the polarized HA powder was significantly higher than that of non-polarized HA, for both of the adsorbate and pH used in the evaluations. The electrically polarized HA powder could improve the performance of column chromatography techniques.