The Effect of Immunotherapy and Chemotherapy upon Mouse Neuroblastoma

Abstract

The blood of the A/J mouse, sensitized by BCG and tumor cells in various ways, was repeatedly transfused to the tumor bearing mouse. The anti-tumor effect of this treatment was studied. As the first step of sensitization, 1, 000μg of BCG was intravenously injected to the untreated mouse. The second step of sensitization was performed according to the following three methods, during 2 weeks thereafter. 1) Subcutaneous separate injections of BCG (250μg) and the tumor cells (3×10^5) preserved at -80℃. 2) Subcutaneous separate injections of BCG (250μg) and the live tumor cells (2×10^4). 3) Simultaneous subcutaneous injection of a mixture of BCG (250μg) and the live tumor cells (2×10^4). The sensitized fresh blood of 0.2 ml, drawn from the mouse 2 weeks after second sensitization, was daily injected to the tumor-bearing mouse for 10 days begun at 2 days the transplantation. The following results were obtained 1) The blood sensitized by the tumor cells preserved at -80℃ gave an effect of shortening the survival term for about 4 days in comparison to the contrast (p<0.05). 2) The blood with combined sensitization of BCG and live tumor cells provided a marked delay of the tumor appearance for 18 days (p<0.001) and prolongation of the survival term for 10 days (p<0.05). The effective sensitized blood transfusion was combined with a chemotherapy of Adriamycin and Cyclophosphamide, begining at 10 days after the tumor transplantation. The survival term of the group with combined treatment was prolonged for about 10 days in comparison to the contrast group (p=0.07).