Abstract
Stage IV-S neuroblastoma is considered especially as immunological relation in terms of spontaneous regression, differentiation, and remission. Peripheral blood lymphocytes (PEL) from Stage IV-S neuroblastoma can kill specifically human neuroblastoma cell lines (NB 1, NBGOTO, TN-1), and it does not kill human malignant melanoma cell line (P 39), or murine neuroblastoma cell line. (C-1300) The cytotoxic activity is markedly depressed by the treatment of PEL with anti-human Leu-1 monoclonal antibody plus rabbit complement. Furthermore, the activity of CTL is specifically blocked by anti-human Leu-2a monoclonal antiboby, but not by anti-human Leu-3a monoclonal antibody. This fact suggests that Leu-2a molecules which have been considered to be carried by human cytotoxic/suprresor T lymphocytes have responsivility for cell-mediated cytotoxicicty. We examined the kinetics of CTL in Stage IV-S neuroblastoma for three months. Surprisingly, we could detect CTL during preoperative and postoperative periods, in spite of tumor bearing condition. Furthermore, her mother had CTL, too, as level as patient. This experiments suggest that Stage IV-S neuroblastoma has high level CTL everytimes, and dominantly hereditary relation to immunology.
