Abstract
For the purpose of finding a cause of the neonatal gastric rupture, we performed an experiment. An ischemic gastric re-perfusion method was prepared using rabbit. We assessed 1) a correlation between the effects of ischemic re-perfusion and the gastric mucosal disorder, 2) an influence of milk and buffer solution as gastric contents consists of group I--14% milk, groupII--pH8.0 McIlvain buffer solution and group III--pH2.5 McIlvain buffer solution. Results were as follows : 1. After ischemic re-perfusion technique, an increase of substances related to active oxygen and elevated intensity of ESR (electron spin resonance) signal were observed. We considered that the free radical had casused the histological disorder on the stomach. 2. Epithelial cells and mucous-producing cells were disappeared during the experiment. These are the protective factors for the gastric mucosa. The parietal cells and chief cells were remained. These cells are well known to produce gastric acid and secrete digestive enzyme. 3. When McIlvain buffer solution (pH 2.5) was administered after ischemic re-perfusion, no increase of gastrin, mucosal blood flow or substances related to active oxygen was observed. 4. When McIlvain buffer solution (pH 8.0) or 14% milk was administered after ischemic re-perfusion, increases of gastrin and secretion of gastric acid were noted. It caused an exacerbation of mucosal lesions. 5. A sharp increase of gastric mucosal blood flow was observed in the milk-administered group. It was presumed that severer disorder was brought about by newly developed active oxygen. A hypoxic state are often seen in neonates at the perinatal period. This seems to be a mimicing of ischemic re-perfusion state of this experiment. Therefore, from the above, the possibility was suggested that the ischemic re-perfusion disorder presumedly occur at the recovery of hypoxic state. A suckling constitute parts of the causes for neonatal gastric rupture.
