Abstract
Purpose. We newly developed a less invasive bronchoscopic microsampling (BMS) probe to assess biochemical substances in epithelial lining fluid (ELF). We have been reporting that measuring biomarkers in ELF was useful for diagnosis of pulmonary diseases in clinical setting. In the present study, we examined whether if proteomic analysis of ELF by BMS method is possible. Methods. 1) ELF in Lipopolysaccharide (LPS) induced lung injury model was collected using BMS probe specially designed for rats. 2) The BMS probe was inserted through a bronchoscope channel and regional ELF was collected selectively in patients with lung cancer. Two ul of ELF was analyzed by SELDI-ToF-MS (Proteintip System^<TM>, Ciphergen Biosystems) in both experiments. Results. 1) Increasing TNF-α level of ELF (ELISA method) in LPS rat model had been confirmed. Some peaks (31000,47000,63000 Da) were detected in LPS rats. These peaks were not detected in control rats and rats which were treated by TNF-α antagonist 2) Several peaks (3460,9280, 20800, 5230 Da) were detected in ELF that was collected from tumor side in patients with adenocarcinoma compared to contralateral side. Conclusion. Proteomic analysis of ELF by BMS method was possible. (JJSRE. 2006;28:592-596)
