Abstract
Primula cuneifolia Ledeb. var. cuneifolia occurs in alpine regions of Hokkaido. It is described as rare species in the Red Data Book of Hokkaido because the individuals density is low anywhere. In this study, we attempted to determine the genetic variation of the species as useful information for conservation and restoration. Two natural populations of the species in Rausuko and Furanodake, were investigated to sequence two chloroplast DNA regions of trn L-trn F and atp B-rbc L. Consequently, some genetic variations were detected between the studied populations, not within the populations. This result suggests that the both populations are in a state sensitive to environmental changes.