Abstract
High blood viscosity is a factor of cause of various thrombotic disease. The blood viscosity changed by Ht level and flexibility of RBC. And their factors has influence on the blood circulation.
In this study the in vitro effects of tocopheryl acetate and tocopheryl nicotinate on the blood viscosity, RBC 2·3 diphosphoglycerate (DPG), RBC fragility and flexibility of RBC in fresh blood and stored blood were investigated.
The visconic EL type viscometer for blood viscosity, a coil planet centrifuge (CPC) for osmotic fragility of RBC an enzymatic method for DPG content of RBC, low shears rate viscosity of packed cells and nucleopore filter with pore size 5μ for flexibility of RBC were employed and measured respectively.
Both tocopherols were added over 50/ml in fresh blood had inhibitory effect on increasing blood viscosity and, on decreasing fragility and flexibility of RBC during preservation. But both tocopherol could not only inhibited on decreasing DPG level of erythrocytes during storage.
On the otherhand, tocopherol added in blood over 14 days after preservation. was not recovered blood viscosity, fragility and flexibility of RBC injured during preservation.
Namely, tocopheryl acetate and tocopheryl nicotinate were inhibition of lipid peroxidation in erythrocytes membrane, maintenance of RBC flexibility in young RBC and blood viscosity in fresh blood, but no effect in aged RBC and in stored blood over 2 weeks.
These result strongly suggest that tocopheryl nicotinate and acetate bring to improvement of blood circulation due to act not only upon platelets and vessel but also upon erythrocytes.
