Abstract
Scanning electron microscopic observation of morphological change of platelets when calcium (Ca) was added to platelet-rich plasma (PRP) was carried out in healthy, patients with hemophilia and thrombocythemia.
1) In PRP, many platelets showed pseudopods or aggregation more or less. This may be attributed to stimulation on platelet at blood collection and centrifugation. But platelets with severe abnormality in thrombocythemia showed on pseudopods at all. 2) When fluid was moved after addition of Ca to PRP, platelets was transformed physiologicaly and showed “cherry blossom-like” in generaly. 3) In the case of hemophilia, fibrin-nets were produced independently from the platelets or products of transformed platelets. 4) Morphological change of platelets when Ca was added to PRP of thrombocythemia were multifariousness. When Ca was added to PRP of thrombocythemia, moved and fixed at a proper good time, a few fibrin-like markedly long pseudopods were seen in platelets.
Even morphological changes that could not be recognized in normal could be inferred by using PRP of hemophilia and thrombocythemia.
