Abstract
Fibrin polymers formed from fibrinogen with thrombin in the presence of EDTA were suspended in a medium containing glucose, arabic gum and imidazole-HCl buffer and were sonicated at 20kHz for 20min to make a suspension containing fibrin particles of small size. The fibrin suspension was used as a substrate for determining the enzymic activity of plasmin and plasminogen activated with urokinase. The kinetic study on the reaction of the fibrin particles with plasmin in the presence and the absence of fibrinogen revealed that the Km value of fibrin for plasmin is 4.2×10-7M and the Ki value of fibrinogen is 1.2×10-5M.
