Abstract
Wound healing is very imortant in surgery.
In the process of wound healing, the development of granulation tissue is necessary, and here the enhancement of insoluble fibrin is essential.
As development of the insoluble fibrin clot plays an important role in the process of wound healing, we carried out studies to immobilize thrombin and Factor XIII on absorbable gelatin sponge and suture materials, which would hopefully enhance the local accumulation of fibrin.
A gelatin sponge sheet (5×2.5×0.5cm) was immersed into a solution containing thrombin and Factor XIII and then it was lyophilized. DEXON (polyglycolic acid gut) and Cat gut were also treated by the same methods. Nylon gut had been dehydrolized with 3N HCl and then thrombin and Factor XIII were immobilized on the surface.
Each material was incubated in normal human plasma to test the activities of the proteins immobilized.
Fibrinogen levels and FDP in the plasma were measured before and after the incubation. Fibrinogen levels were detected by the method of turbidimetric analysis and FDP, by Latex coagulation method. All materials were studied histologically. Non-treated materials were served as controls.
The fibrinogen levels in the plasma incubated with the immobilized materials decreased remarkably and the FDP levels varied.
The histology showed that a large amount of fibrin had accumulated on the treated materials, however, no fibrin formation was observed on the controls.
In vivo study is now underway.
