Abstract
Larger amounts of FDP were found in the ascitic fluid associated with peritonitis carcinomatosa than in the non-malignant fluid. By Todd's fibrin slide method, the dissolution of fibrin was observed around free cancer cells in the ascites, but not around the granulocytes. Therefore, it was suggested that the origin of FDP in malignant ascitic fluid was the plasminogen activator dependent on the cancer cells.
By Lysine-Sepharose affinity chromatography, plasminogen activator was partially purified 4, 500 folds from ascitic fluid with peritonitis carcinomatosa of breast cancer (FDP 1, 280μg/ml). Euglobulin fraction of the malignant ascites was applied to the column on Lysine-Sepharose 4B, and eluted with 0.5M NaCl, 1M NaCl and 0.1M acetic acid. The activator was observed in the fraction of 1M NaCl elution, and plasmin in the fraction of 0.1M acetic acid.
On Ouchterlony's method, a precipitin line was not observed between the activator and anti-urokinase rabbit serum.
This result indicates that this plasminogen activator might be different from urokinase.
