Abstract
The Chandler's loop technique of experimental “thrombus” formation in vitro was employed in the present study. Clot weight was measured at 9 A. M., 3 P. M., 9 P. M., 3 A. M, and 9 A. M., consecutively, in 4 control subjects and 7 patients with atherosclerosis. The Control subjects showed constant results: the lowest weight was 53.7±6.2mg (M±SE) at 3 A. M., and the highest 64.7±6.4 at 3 P. M. The patients gave 70.6±6.2 at 9 P. M., and 116±13.6 at 3 A. M., significantly higher, revealing distinct diurnal change, while the patients produced heavier clot than the control subjects in each of the five determinations. Experimental clot was also weighed in 6 younger subjects, 10-29 of age, 13 middle-aged, 30-59, and 13 older, age over 60, monthly in January through December. Seasonal fluctuation in clot weight was not found out statistically. The mean in 12 months ranged 39.4-52.5mg, 60.5-87.2, and 58.0-101.5 in younger, middle-aged and older subjects, respectively, showing broader distribuation of the value with the progression of age. The data might be correlated to a pathophysiological aspect in the development of thrombosis and atherosclerosis.
