Blood & Vessel
Online ISSN : 1884-2372
Print ISSN : 0386-9717
Culture of human endothelial cell and platelet aggregation
Kenji KAWAGUCHIYasuhiro ORIBEHaruo UZAWA
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JOURNAL FREE ACCESS

1981 Volume 12 Issue 4 Pages 607-609

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Abstract
Human endothelial cells were cultured from umbilical cord veins using Gimbrone's method. The cultured cells were identified as endothelial cells by morphology, growth pattern and the presence of Weibel-Palade bodies. After 5 minutes incubation of platelet rich plasma with endothelial cells, ADP-induced platelet aggregation (10μM) was completely inhibited. 2×105 cultured endothelial cells indicated the same inhibitory effect on platelet aggregation as the effect of authentic prostacyclin, 10ng/ml. This inhibitory effect was completely blocked after the addition of aspirin (5μM) on the cell culture medium.
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© The Japanese Society on Thrombosis and Hemostasis
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