Abstract
The arterial thrombus was made experimentally in rabbit carotid arteries by the methods of both denudation of the endothelium and stenosis of the lumen.
A Fogarty balloon catheter (2F or 3F) was inserted in the common carotid artery and balloon was inflated by air at the bifurcation, that was pulled back gently to the proximal portion. Then inserted portion was by-passed by asiliconized polyethylene-tube. Immediately, the carotid artery with the de-endothelialization was ligated with a steel wire (0.5mm) at the distal portion, and was stenosed by pull out of the wire.
Another carotid artery treated only de-endothelialization or luminal stenosis by the same methods. The artery with only endothelial denudation showed marked platelet adhesion and aggregation at the subendothelium, but never formed any thrombus at 30 minutes. The artery with only luminal stenosis formed platelet thrombus in 80% at the site of the ligation, but the normal endothelium did not showed either platelet adhesion, thrombus formation, nor the luminal obstruction.
The combined treatment of the de-endothelialization and the luminal stenosis, a thrombus was formed in 100% and luminal obstruction was observed in 78% of the studied arteries at 30 minutes. The thrombus was formed at the distal portion and protruded the head to down stream over the stenosed portion, and it was accompanied by a coagulation thrombus.
These results suggested that platelet adhesion and aggregation occured by the endothelial injury, and combination of the arterial luminal stenosis might be necessary to arterial obstructive throinbus formation.
