1986 Volume 17 Issue 2 Pages 119-126
Automated assay method for purified tissue type plasminogen activator (tPA) was established which used a flexible centrifugal auto-analyzer COBAS FARA (F. Hoffmann-La Roche & CO.) and a chromogenic substrate S-2288. A release of the product, p-nitroaniline was continuously monitored by the absorbance at 405nm and the data obtained were analyzed by a computer-aided processor. When compared with the conventional assay methods reported, this method has several advantages. The time required for the measuring and data-analysis is very short (10min). The sensitivity is high; a small quantity of sample can be measured (10μl) with less amount of the expensive substrate, S-2288 (26μM).
