Abstract
We reported that thrombokinetic study was useful to detect early stage of platelet plug formation and adhesion in vivo on the cases of thrombocytosis Blood and Vessel 8 (2), 193-198, 1977. Afterward we added more cases with risk factors of thrombogenesis and discussed about their significance by means of throm bokinetic study.
Clinical studies. A total of 28 cases were studied, composed of 24 cases of cardio-valvular replacement, 2 cases of vascular disorders and 2 cases of diabetes mellitus with retinopathy. The result of this study showed that platelet survival in cases without thrombosis was almost normal but was significantly shortened in cases with thrombogenic episodes (2 cases of chronic myelogenous leukemia (CML), 1 case of myelofibrosis with myeloid metaplasia (MMM), 1 case of primary thrombocythemia). The shortened platelet survival time implied increased platelet consumption for thrombus formation.
We measured von Willebrand factor activity, and FVIII related antigen (FVIII AGN) in cases of vascular disorders (34 cases of diabetes mellitus with retinopathy, 10 cases of hypertension). Diabetes mellitus with retinopathy were proved to have high vWF activity and high VIII AGN value. On the other hand, hypertension with retinopathy showed high vWF activity but normal FVIII AGN value.
In 2 cases of diabetes mellitus with retinopathy, platelet survival time was significantly shortened.
These results suggested that increased FVIII AGN might initiate and produce vascular change and resulted in platelet consumption and thrombus formation.
Experimental studies.
SHR (spontaneously hypertensive rat) was used as the experimental model of hypertension. We studied platelet survival time on spontaneously hypertensive ratstroke prone (SHR-SP) and spontaneously hypertensive rat-stroke resistant (SHRSR) by means of in vivo labelling with 75Se-Selenomethionine.
The result of this study showed that significantly short survival was observed in SHR-SP of 10 weeks of age compared with SHR-SR.
Platelet seems to have some concern with elevation of blood pressure and producing vascular alteration on SHR-SP.
