Influence of Blood Flow in the Intravascular Thrombus Formation
In vivo Study using Hybrid Vascular Model
1995 Volume 6 Issue 2 Pages 105-118
Details
1995 Volume 6 Issue 2 Pages 105-118
Influence of blood flow in the intravascular thrombus formation was evaluated in vivo study using cultured endothelialized glass tube vascular model and the in vitro study using the flow visualizing model (FVM). 1) 1.5×50mm straight silica glass tube were used as a physiological hybrid vascular model (P-HVM). Endothelialization were performed by rotator cultivation using bovine aorta endotherial cells suspended in RPM1 1640. The femoral artery and vein of eighteen mongrel dogs were interposed by the model respectively. The model were exposed to blood flow for 1 to 24 hours. The average blood flow of femoral artery and vein were both 30ml per minute and not significant dif f errence between artery and vein in blood flow was found. Arterial replacement P-HVM demonstrated 100% patency for 24 hours. SEM micro graphs showed a fairly clean surface morphology with minimal platelets adhesion. In contrast, the longest patency of P-HVM in the vein was 18 hours, and line shaped thrombus was observed inferior area 1 hour after replacement. 2) 2.0×50mm tube which has eccentric dilatation or stenosis in the center were used as pathological model (D-/S-HVM). After endotheliazation, the model were interposed. FVM was developed using open circuit which connected with the artificial heart and scale up model of D-/S-HVM. The correlation between in vitro hydrodynamic events and in vivo results were evaluated. FVM demonstrated the stagnation in the dilatation area of D-HVM and peripheral area of the stenosis in the S-HVM. Initial thrombus formations in vivo model observed just on the flow stagnation area of FVM. It would be concluded that the difference in the shear stress between artery and vein on the vessel wall leaded to the difference of the thrombus formation in P-HVM. The shear stress diminution on the vessel wall by flow disturbance from dilatation or stenosis would cause of initial intravascular thrombus formation.
