CONSECUTIVE MONITORING OF DISSOLVED OXYGEN CONSUMPTION BY DOX^TM SYSTEM TO EVALUATE BACTERIAL CONTAMINATION IN PLATELET CONCENTRATES

Abstract

Background: Transfusion-transmitted bacterial infection is a serious and unresolved problem. We evaluated the efficacy of DOX^TM (Daikin Industries) which is a commercially available system that has developed to detect a contaminated food by measuring oxygen potential to detect bacterial contamination in PC.
Methods: Staphylococcus aureus, Serratia marcescens, Staphylococcus epidermidis, Bacillus cereus, Streptococcus pneumoniae, and Propionibacterium acnes were inoculated into PC at a final concentration of approximately 10⁰, 10¹ and 10² CFU/ml. Dissolved oxygen potential of the sample taken from each PC just after inoculation was measured consecutively.
Results: The detection rate by DOX^TM system in samples inoculated with S. aureus at a concentration of 10⁰, 10¹ and 10²CFU/ml was 28.6%, 78.6% and 85.7%, respectively. That of S. epidermidis was 23.0%, 84.6% and 92.3%, of S. marcescens was 50.0%, 100% and 100%, and of B. cereus was 57.1%, 100% and 100%, respectively. The aerobic organisms were detected in a mean of 7.0 to 18.2 hours.
Conclusion: DOX^TM system detected aerobic bacteria in PC within 20hours if their initial concentration was more than 10¹CFU/ml. We consider that this system can be introduced as a useful method to detect the contaminated PC, and can improve the platelet transufusion safety without increasing the medical costs.