Japanese Journal of Transfusion and Cell Therapy
Online ISSN : 1883-0625
Print ISSN : 1881-3011
ISSN-L : 1881-3011
Case Reports
A CASE OF SUSPECTED PARTIAL D BECAUSE OF WEAK REACTIVITY TO ANTI-D ON COLUMN AGGLUTINATION TECHNOLOGY AND IDENTIFIED AS PARTIAL D (DBT-1) BY GENETIC TESTING
Yukari NishiyamaKumiko IzumidaMisae KinoshitaTomoko FuruyaYoko YoshiuraHironobu KawashimaAkira MatsunagaHiroshi IdeguchiTomoko TakuboIwane SakotaYoko TomonariHiroyuki SatoHiroyuki KiyokawaMitsunobu TanakaJunko TakahashiYoshihiko Tani
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2011 Volume 57 Issue 4 Pages 267-273

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Abstract
A 58-year-old man was admitted to our hospital because of cerebral hemorrhage. Blood type testing on admission with the automated instrument AutoVue Innova® showed weak reactivity to anti-D, indicating the possibility of weak D or partial D. Further examinations using anti-D polyclonal and monoclonal antibodies showed a similar reactivity pattern as for the partial D category DBT phenotype.
The genomic DNA analysis by polymerase chain reaction with RHD exon-specific primers revealed no amplification of exons 5 to 7, and the following cDNA analysis suggested exons 5 to 7 of the RHD gene were replaced by RHCE equivalents. Thus, the patient was identified as DBT-1 (RHD-CE (5-7)-D).
Reactivity of the anti-D by column agglutination technology is generally strong compared to that of the tube method. It is therefore important to keep in mind the possibility of weak D or partial D if the reactivity of the anti-D by column agglutination technology is weaker than 3+.
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© 2011 The Japan Society of Transfusion Medicine and Cell Therapy
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