Abstract
It has been reported by some studies that unstable coagulation factor activities in thawed fresh frozen plasma (FFP) decreased with time. However no report has evaluated quality change in repeatedly freeze-thawed FFP. We assessed the quality of FFP derived from 400 ml of whole blood over three freeze-thaw cycles. Regarding activities of Factor (F) V, F VIII activities were decreased, and APTT was prolonged by repeated freeze-thawing, but no significant changes in pH, sodium concentration, potassium concentration, PT, fibrinogen concentration, activities of F II, F VII, F XI, VWF: RCo were noted. PT after three freeze-thaw cycles was 11.3±0.7 sec, which meets the coagulation examination standard in "Minimum Requirements for Biological Products" (less than 20 sec). Activity of F VIII after three freeze-thaw cycles remained at 0.72±0.23 IU/ml, which satisfied the European guideline (more than 0.70 IU/ml). Thrombin generation time might be prolonged by repeated freeze-thawing, but peak height and endogenous thrombin potential were not significantly reduced by freeze-thawing repeatedly.
