新鮮凍結血漿の長期保存後の凝固因子活性

渕崎 晶弘; 森 純平; 岩間 輝; 柴 雅之; 内藤 祐; 林 宜亨; 秋野 光明; 松本 真実; 益子 毅; 小野寺 秀一; 金子 祐次; 榎本 圭介; 茶谷 真; 栗原 勝彦; 小池 敏靖; 寺田 あかね; 大橋 祥朗; 佐竹 正博; 田所 憲治

doi:10.3925/jjtc.62.545

Originals

ACTIVITIES OF COAGULATION FACTORS IN FRESH FROZEN PLASMA AFTER LONG-TERM STORAGE

Akihiro Fuchizaki, Jumpei Mori, Akira Iwama, Masayuki Shiba, Yu Naito, Yoshiaki Hayashi, Mitsuaki Akino, Mami Matsumoto, Tsuyoshi Mashiko, Hidekazu Onodera, Yuji Kaneko, Keisuke Enomoto, Makoto Chatani, Katsuhiko Kurihara, Toshiyasu Koike, Akane Terada, Yoshio Oohashi, Masahiro Satake, Kenji Tadokoro

Author information

Akihiro Fuchizaki
Japanese Red Cross Society, Blood Service Headquarters, Central Blood Institute
Jumpei Mori
Japanese Red Cross Society, Blood Service Headquarters, Central Blood Institute
Japanese Red Cross Kinki Block Blood Center
Akira Iwama
Japanese Red Cross Society, Blood Service Headquarters, Central Blood Institute
Masayuki Shiba
Japanese Red Cross Society, Blood Service Headquarters, Central Blood Institute
Yu Naito
Japanese Red Cross Hokkaido Block Blood Center
Yoshiaki Hayashi
Japanese Red Cross Hokkaido Block Blood Center
Mitsuaki Akino
Japanese Red Cross Hokkaido Block Blood Center
Japanese Red Cross Society, Blood Service Headquarters
Mami Matsumoto
Japanese Red Cross Kanto-Koshinetsu Block Blood Center
Tsuyoshi Mashiko
Japanese Red Cross Kanto-Koshinetsu Block Blood Center
Hidekazu Onodera
Japanese Red Cross Kanto-Koshinetsu Block Blood Center
Yuji Kaneko
Japanese Red Cross Kanto-Koshinetsu Block Blood Center
Keisuke Enomoto
Japanese Red Cross Kanto-Koshinetsu Block Blood Center
Makoto Chatani
Japanese Red Cross Kanto-Koshinetsu Block Blood Center
Katsuhiko Kurihara
Japanese Red Cross Kanto-Koshinetsu Block Blood Center
Toshiyasu Koike
Japanese Red Cross Kinki Block Blood Center
Akane Terada
Japanese Red Cross Kinki Block Blood Center
Yoshio Oohashi
Japanese Red Cross Kinki Block Blood Center
Masahiro Satake
Japanese Red Cross Society, Blood Service Headquarters, Central Blood Institute
Kenji Tadokoro
Japanese Red Cross Society, Blood Service Headquarters, Central Blood Institute
Japanese Red Cross Society, Blood Service Headquarters

Keywords: FFP480, FFP240, Activity of coagulation factor, Thrombin generation, Freeze preservation

JOURNAL FREE ACCESS

2016 Volume 62 Issue 4 Pages 545-551

DOI https://doi.org/10.3925/jjtc.62.545

Details

Abstract

Japanese guidelines for usage of blood products for transfusion describes prothrombin time (PT), activated partial thromboplastin time (APTT), and activities of Factors (F) II, V, and VIII in FFP. However, no studies in Japan have reported the activities of other coagulation factors. We investigated the activities of 15 coagulation-related factors in apheresis-derived and whole blood-derived FFP (FFP480 and FFP240, respectively) after 1, 3, 6, 9, 12, and 13 months of storage.

Only FVIII showed >10% decrease in activity after storage in both FFP480 and FFP240. Activities of all other coagulation-related factors showed high stability during storage. All samples met the Japanese standard in "Minimum Requirements for Biological Products" (PT ≤20 sec). All parameters in thrombin generation tests showed ≤10% change after storage. Although O-group samples showed lower FVIII and VWF: RCo levels than non O-groups, they had thrombin generation activity comparable to non-O-groups.

Corresponding author

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