2020 Volume 66 Issue 3 Pages 524-530
This study compared test methods for detailed examination of blood type after ABO-incompatible cord blood transplantation. Of the 72 subjects who underwent transplantation between May 2016 and October 2018, 34 had a major mismatch, 25 had a minor mismatch, and 13 had a major/minor mismatch. After transplantation, the first follow up was performed in those with no history of transfusion in the previous 3 months. Conversion to the donor blood type was confirmed by forward cell grouping, changes in antibodies by reverse grouping, and loss of recipient antigens by adsorption elution tests using column tests (C-tests) and test-tube tests (T-tests). Furthermore, ABO genotyping and an intergroup comparison were performed. T-tests are superior for detecting the sensitivity of partial agglutination, while a problem with C-tests is that the nozzle of the automatic machine does not accurately sample new donor blood cells with low specific gravity. The adsorption-elution test revealed remnant recipient antigens in 19 cases (50.0%), indicating missed timing for conversion to the donor blood type. While genotyping showed conversion to the donor blood type in all cases, the results obtained using other methods differed, which was concerning. We conclude that blood type examination after transplantation can be performed using T-tests alone.
