FACTORS PREDICTIVE OF CD34-POSITIVE CELL YIELD IN PERIPHERAL BLOOD STEM CELL COLLECTION FROM HEALTHY DONORS

Abstract

In allogeneic peripheral blood stem cell transplantation, it is essential to accurately predict stem cell yield before collection in order to minimize burden on the donor and to ensure optimal cell dose for recipients. Therefore, we retrospectively examined allogeneic peripheral blood stem cell collection in 92 healthy donors, and analyzed factors that affect stem cell collection yield. Donors included 32 women (34.8%), and median age and platelet count before G-CSF administration were 41.5 years (14-61) and 24.7 × 10⁴/μl (13.9-41.0). Median ratio of actual G-CSF dose to the standard dose according to the package insert was 96.3% (56.4-109.0). Median blood throughput and CD34⁺ cell yield on day 1 of collection (day 4 of G-CSF initiation) were 10 l (4.5-18) and 1.96 × 10⁸ cells (0.43-6.23), and 14 patients (15.2%) required collection for at least 2 days. Older age, lower platelet count before starting G-CSF administration, and lower G-CSF dose in donors were associated with a significantly reduced yield of CD34⁺ cells. Our results suggest the importance of administering an adequate dose of G-CSF, and that reliable yield prediction based on pre-collection factors may help reduce the donor burden by preventing extension of the collection schedule.