Evaluation of the Influence of Universal Buffer Solution on Diffusion Phenomena of Protein

Abstract

In this paper, the influence of universal buffer on diffusion phenomena of protein was studied. We have developed short-time yet precise observation system of mass diffusion phenomena of protein. The system can produce spatially clear and high resolution concentration distribution image compared with conventional interferometer. In order to investigate the influence of pH value of the buffer on diffusion phenomena, several pH values of universal buffer solution were used. Also, to investigate the concentration dependency of pseudo mass diffusion coefficient, solutions over a wide range of concentration were prepared. The experiments were carried out under isothermal condition, and available data were found. The dependency of pH value of surrounding buffer and that of concentration on diffusion phenomena were discussed.