Abstract
Six newborn Japanese Black calves with severe hemolytic anemia were noted from 1991 to 1993. All calves showed typical spherocytosis in which most of the cells were microcytic spherocytes deprived of the central pallor, and it was also clear that osmotic fragility of their red cells was significantly increased. Autoimmune disorder, enzymopathies disorder. red cell membrane protein were examined, to investigate abnormalities of Red cells. In consequence it was made clear that 6 cases were "Red blood cell membrane protein abnormality" with band-3 deficiency. It was clear that, hereditary spherocytosis in human was occurred in autosomal dominant transmission. Red cell membrane protein deficiency in Japanese black cattle. To reveal that these cases were occurred in hereditary, clinical test, blood and biochemical examinations, shape of red cells, osmotic fragility test and analysis of membrane proteins were performed in 6 dams and 15 non-consanguinty calves. There was significantly higher number of spherocyte in the 6 dams as compared to the non-consanguinty calves. There was significantly mild increased osmotic fragility in the 6 dams compared to the non-consanguinty calves. Osmotic fragility of dams were significantly higher than that of non-cosanguinty normal cattle in a rate of hemolysis in 0.5% NaCl at fresh and 24 hour incubated blood (p<0.01). Particularly, to compare the existent ratio of band-3 and 4.2 in each groups, existent ratio of band-3 and 4.2 in dams showed 63%, 80% respectiely. It was made clear that those of dams developed middle value between affected calves and non-consanguinty calves. Based on their findings, mode of hereditary was examined. It 6 affected calves (band-3 deficiency) were Homotype (AA), dams and a part of siblings (middle values of band-3) were Hetrotype (Aa), non-consanguinty calves (complete developement of band-3) were non-carier type (aa), these cases were determined as "Autosomal incomplete dominant transmission" in all cases. According to assum genotype used by osmotic fragility test, cows for production in A fame were diagnosed genotype and all cows were mated with bulls assumed non-carrier after control. The effect of these control were observed, according with comparison of genotype of newborn calves during pre-control (1991-1992) and post-control (1993-1994). Frequency of genotype changed form 54% to 22% before and after control. When genotype of cows and bulls were diagnosed respectively, possiblity of hereditary control was proved in clinical field.
