Abstract
The purpose of this study is to evaluate the environmental condition by drug resistant and molecular epidemiological analysis of Staphylococcus isolated from raccoon dogs and Japanses Black bears. Nineteen out of 26 raccoon dogs were isolated S. delphini and ten out of 35 bears were isolated S. aureus and S. warneri A respectively. Eight out of 26 wild raccoon dogs have staphylococci that were resistant to at least one of the drugs tested. Six out of 35 wild bears have resistant staphylococci. The genomic DNAs of 37 staphylococci of raccoon dogs and bears were cleaved with restriction endonulease SmaI and subjected to pulsed-field gel electrophoresis. Two strains of S. sciuri isolated from two bears of the near areas were shown a same DNA pattern. It is indicted that these two strains of tetracycline resistant were infected from human to bears.