Abstract
The final aim of this study is the development of Japanese serow's individual group management method and grasp of present condition. Takizawa forest (Field Science Center, Faculty of Agriculture, Iwate University) is the isolated forest of Morioka City suburb. In 1980's, Japanese serow of 8-27 heads inhabited in the forest. However, in the present, the surrounding environment of its forest changes by the construction of a new railway station etc. Therefore, it is thought that the genetic background and habitat environment of them changes, too. In this study, we researched about wild animal medicine (blood type, DNA individual identification, parasites) and ecology (inhabitable number, food property, territory and behavior) of the Japanese serows inhabiting Takizawa forest. As the results, we observed seven heads and succeeded to capture one of them. He inhabited in 10 ha territory with mother until two years old. But after that, he had a own territory left 2 km from mother's territory. As for the serum protein polymorphism of Japanese serow which have inhabited in the suburbs of Morioka-shi, the serum albumin and transferrin in particular, two types and six types were observed. DNA collected from feces were amplified with PCR. The banding pattern from 200 to 300 by of PCR products were a one band (Amelogenin gene XX) for female and three bands including a miner band (Amelogenin gene XY) for male. Finally, the probability of sexing from feces using this method was 95%. It was indicated that this method was effective for sexing using feces piles (Tamefun in Japanese). In addition, many parasites were observed in these Japanese serow. The wild animal medical research must continue by many researcher's cooperation.
