Abstract
Breeding seasons in animals are influenced by temperature, potentially leading to shifts in timing along latitudinal gradients. To accurately determine the breeding season, it is essential to collect data on reproduction across various regions. In early May 2024, we observed mating-related behaviours of the Japanese giant flying squirrel (Petaurista leucogenys) on Kyushu Island, the southernmost habitat of this species. The mating dates at this study site (32°49′N) were estimated to be from May 5 to 7, which is approximately one week earlier than results of 14-years observation in two populations (34°41′N -35°35′N) on Honshu. In order to investigate the relationship between the mating days of the flying squirrels and temperature in more detail, it is necessary to gather more information on mating in Kyushu, as well as those in habitats further north, such as the Tohoku region.