Studies on the Lysis-Phenomenon of Leucocytes

Rep. IV. Experiment with Anti-Leucocyte-Serum

Abstract

After a certain time following to the intra-peritoneal injection of physiologic saline solution to the rabbits, the peritoneal fluid was collected and physiologic saline solution containing 5% leucocyte suspension was prepared by means ofgathering leucocytes in that peritoneal fluid.2.0 cc of this solution were injected intraperitoneally to quinea pig every other day, five times. Serum was separated from the blood taken out entirely from the animals on the 10th day from the last immunization. The lytic activity of thus obtained anti-rabbit leucocyte quinea-pig-serum to the rabbit leucocytes was observed. Results were as follows:
1) Anti-leucocyte-serum showed agglutination titer of 8650×.
2) Anti-leucocyte-serum caused distinct degeneration to peripheral pseudoeosinophiles, and the cells going to complete lysis were recognized.
3) Anti-leucocyte-serum is stable at 56.0°C for 30 minutes, and necessitates the existence of the complement.
4) Anti-leucocyte-serum did not influence myeloblastes, promyelocytes were scarcely influenced, some of myelocytes were recognized slightly affected, and metamyelocytes were influenced to some extent. In another word, higher the maturity of the cells, the more of them were influenced.