Abstract
In an attempt to study the mode of cancer spreading to extravascular fluid paths in rabbits the following experiments were performed.
1. A suspension of Brown-Pearce cancer cells was injected into the testis and muscles. Each rabbit was sacrificed 2 to 4 weeks after injection, for histological study. Cancer depowere observed in the macula cribriformis of the diaphragm and in the fatty tissues around internal mammary lymphatics. These cancer deposits showed a slight gradual increase in number. These findings seems to support a view that cancer spreads through extravascular fluid paths.
2. Rabbits which were inoculated intraabdominally with a suspension of Brown-Pearce cancer cells were exsanguinated (10/kg.3 times, at 2 day intervals). They all demonstrated a tumor cell infiltration at the diaphragm, and the adipose tissues around internal mammary lymphatics, as well in the lungs and the liver. The tumor cell infiltration was observed in metastatic foci with corresponding histolgical changes. Exsanguinated rabbits were more easily metastasized than non exsanguinated ones.
