Especially on the precipitin produced in its Blood

Abstract

According to our serological studies on the crawfish, Cambarus clarkii, Crustacea, precipitin is demonstrated in the serum of the creature which was injected with 0.2-0.3 cc of rabbits' or goat's serum even just one hour before. This precipitin may reasonably be considered as the specific antibody, because of its being completly absorbed by the tanned cells coated with the homologous (goat's) serum proteins. Moreover it is shown that the precipitin coexist with the injected antigen (serum) in the crustacean blood.
In one dimensional cross paper electrohoresis developing the antigenic serum and its corresponding precipitin serum of the crawfish, when the albumin of the antiserum overlaps the same fraction of the antigen, there can be seen some irregularities of the albumin pattern of the antigen.
As the result of these experiments, it may be considered that the precipitin of the crawfish, extremely different from the mammalian antibody, is contained in the albumin fraction or in some other fractions electrophoretically undistinguishable from the albumin.