The Production of Fluorescent Substances

Pseudomonas aeruginosa

Abstract

Eighteen strains of Ps. aerugincsa were cultured on the glutamic acid liquid medium and the production of the fluorescent substances were examined by the gel filtration of culture fluid filtrate and the paper electrophoresis of fluorescent fractions.
The results are as follows:
1. The common characteristic of all the 18 strains tested was the production of Fluorescence II fraction.
2. In all the instances of pigment producing strains, the yellow or yellowishgreen portion of fluorescent fraction, observed at the early stage of cultivation, may correspond to the Fluorescence I substance.
3. The paper electrophoretic patterns of Fluorescence II fraction of colorless strains (No.58,39 & 65) originated from a pigment producing strain, were quite the same as that of the ordinary colored strains and showed a clear C-spot.
4. In other colorless strains than these, a wide and undistinguishable zone of fluorescence was demonstrated without showing the C-spot distinctly, and the site of C-spot was involved in the wide zone.
5. The aceton soluble fuorescent substances were extracted from the washed cells of both the pigment strains and colorless variants, and the liquid soluble portion which was contained therein was similar to the Fluorescence II substance presented in the culture fluid filtrate.
6. The aceton extracts of bacterial cells of the other colorless strains than these showed only a faint fluorescence.