Abstract
The antibody promoting factor, inosine, in a great amount of quantity has been shown to appear in the circulating blood in the early stage which is called the negative phase after an antigen invasion by our laboratory's reports. Thus the author set out to examine the interrelations between inosine formation and glycolysis in the negative phase. The results obtained were as follows:
1) Inosine formation varied i n contrast to the behaviors of ATP in mouse liver after the typhoid-paratyphoid vaccine (TPV) invasion. The variation was especially remarkable 60 minutes later.
2) Inosine which increased in the spleen was formed mainly in the liver and carried in it, through the circulation. When an adequate glucose was added in a liver homogenate enucleated and made 60 minutes after the TPV invasion and the incubation was continued in vitro for 1 hour, inosine formation was accelerated still more, and pH of the medium gradually turned acidic and fell to pH 6.4-6.5 in the end. NADH decreased slightly and SDH could hardly be discovered in the activity. When the homogenate of mice livers enucleated and made 1 hour after the first TPV invasion was incubated for 30 minutes, the amount of pyruvate in it was shown to increase slightly, also, uric acid increased with the addition of AICA in it. On the contrary, lactate and uric acid in the homogenate of the liver enucleated 1 hour after the second TPV invasion increased remarkably in comparison to the first and the quantity of pyruvate did not increase.
