The Journal of Kansai Medical University
Online ISSN : 2185-3851
Print ISSN : 0022-8400
ISSN-L : 0022-8400
Ultrastructure, and Localization of Electron-microscopic Histochemical Activity of Phosphatases on Mammary Gland and Its Tumorigenesis by 7, 12-DMBA in Female Rats
Part 1. Ultrastructure on Resting Mammary Gland of Adult Female Rats
Kiyoshi Kon
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1970 Volume 22 Issue 1 Pages 81-94

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Abstract
Terminal ductules of the resting mammary glands of young adult female rats were studied by an electron microscope to determine the limits of normal ultrastructural variability.
Fine pieces of the abdominal mammary-fatty tissues of Sprague-Dawley fem a le rats, aged 50-150 days, were packed into a small wire-meshed cago, and then fixed satisfactorily in Karnovsky's fixatives and postfixed in osmium tetroxide buffered with veronal-acetate. Sufficient dehydration in graded alcohols and propylene oxide was followed by embedding in Epon. After the careful trimmings, sections were cut on an ultrathin microtome and stained with uranyl acetate and lead citrate.
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© The Medical Society of Kansai Medical University
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