Preparation and Evaluation of Lipid Emulsion Containing Polynuclear Hydrocarbons

Abstract

Department of Pathology, Kansai Medical School, Moriguchi, Osaka, Japan The preparation, composition, metabolism and clinical application of intravenous fat emulsion have been reviewed by Geyer. Lipomul of the Upjohn Company was studied in animals and man, and it was found to be well tolerated during short-term administration. Recently, experimental lipid emulsion has been prepared as vehicles for parenteral administration of fat-soluble agents. The most notable example is the emulsion containing the carcinogen,7,12-Dimethylbenz (α) anthracene (DMBA), made a popular tool for cancer research. Certain cells are very susceptible to heavy doses of DMBA; in these tissues cancer arises in high yield or invariably and the cancer so evoked are easily demonstrable within several weeks after the first injection. Such an interesting administration of carcinogenic hydrocarbons is named “pulse-doses of carcinogens” by Huggins. The method for preparation of DMBA-emulsion of the Upjohn Company was described by Schurr. Small amounts of the emulsion containing the derivatives of 12-Methylbenz (α)anthracene were prepared by Huggins et al., by means of Lipomul without dextrose.
This communication details the preparation of small amount of the lipid em ulsion containing 34 aromatic hydrocarbons, respectively, and the evaluations of the emulsions prepared.