Abstract
In this experiment, observations were made on the effect of anti-cancer drugs on the growth of chicken embryos as well as on the proliferation of tumor cells cultivated on the CAM.
Re sults:
1) No re markable deviations were found in the weights of the chicken embryos or in those of the cultivated tumors in eggs whose weight range was from 50.0 to 64.9g.
2) As to the anti-cancer drugs examined (5-fluorouracil, cyclophosphamide, cytosine arabinoside, bleomycin, daunomycin, chromomycin A3, and mitomycin C), their maximum toleration doses for 13-14 days old non-tumor eggs were commonly about 1-2 per cent of those usually given to human adults.
3) Embryos of 13-14 days inc ubation were found to be able to activate cyclophosphamide and to inactivate chromomycin A3 as effectively as other experimetal animals.
4) yolk sack injection of the above mentioned anticancer drugs could inhibit the growth of the YS cells cultivated on the CAM, thus yielding results similar to those obtained by the author using the ip (intraperitoneal) - ip system in rats and to those previously reported by other authors.
5) The toxicity of β-methasone to chicken embryos was so remarkable that an evaluation of its anti-cancer effect was impossible to obtain.
6) The above mentioned results testify to the aptitud e of the in ovo sensitivity test for anti-cancer drugs as an adequate in vivo test system.
