1983 Volume 35 Issue 1 Pages 125-146
Alkaline phosphatase activity (AP) and acid phosphatase activity (SP) were histochemically studied in the neutrophils of healthy individua ls and in those of patients with blood disorders, i.e.acute granulocytic leukemia (AGL), chronic granulocytic leukemia (CGL), m yeloma, aplastic anemia (APA), idiopathic thrombocytopenic purpura (ITP), and essential hypochromie (HA). Neutrophils obtained from the peripheral blood or from the hone marrow by puncture were stained by the azo-dye method.
The results obtained were as follows:
1) Immature neutrophils from the bone mar row of normai individuals showed a lower AP and a higher SP than mature neutrophils. Comparing these activities in mature neutrophils from the bone marrow with those from the peripheral blood, the former showed a lower AP and a higher SP thain the latter.
2) In AGL, the AP varied widely, being high in the non-remissive phase and approachiog normal during remission.
3) CGL patients showed a low AP and a high SP in both the peripheral blood and bone marrow neutrophils.
4) In the bone marrow of myelome, the AP of the mature neutrophils was high, while the SP was similar to the control level.
5) In APA, the AP was high both in the peripheral blood and bone marrow neutrophils, whils the SP was normal in the immature neutrophils and high in the mature ones.
6) ITP and HA patients, the neutrophilic AP and SP were similar to those of the normal controls.
