Establishment of Sterile Sorting of NK Cells and Study on their Antineoplastic Effect on Brain Tumors

Abstract

Antineoplastic effect of natural killer (NK) cells obtained through the sterile sorting technique on brain tumor cells was evaluated in order to develop a safe adoptive immunotherapy using NK cells. Following preliminary experiments were performed to establish a sterile sorting technique for NK cells using flow cytometer (FCM): 1) Study on the optimal staining condition for fluorescein isothiocyanate (FITC) -labeled monoclonal antibodies, and 2)Study on sterilization for sterile sorting.