Immunohistochemical Study on the Localization of Serotonin in Platelets

Abstract

Electronmicroscopic immunohistochemical examination using monoclonal anti-serotonin(5-HT) antibody was performed to determine the localization of 5-HT in human and rabbit platelets. The immunological specificity of the platelet membrane, namely, nonspecific adsorption and its impermeability, to antibodies, was resolved by enzymatic treatment of glutaraldehyde-fixed platelets with trypsin. Immunological reactions of anti-5-HT antibody were clearly detected on many α-granules -4. as well as on dense granules. From the manner of these reactions, serial development from α-granules to dence granules was suspected.