1995 Volume 47 Issue 1-2 Pages 55-71
1. Four items. nuclear diamener, N/C ratio, nuclear density and high density area of nucleus were measured as fundamental data necessary for screening. Then, clearly distinguishing differences were observed between normal cell group and abnormal/malignant cell group.
2. Specimens were screened with these 4 items as parameters. The linear di s crimination of the results clearly showed distinction of each cell.
3. Field tests were tried for specimens gener ally utilized in population screening. The rate of correct diagnosis was satisfactory in abnormal group. In normal group, however, the false positive rate came up to 40%. Such factors as cluster of cells, dusts and leucocytes were difficult to be discriminate from abnormal cells by the automatic sieving apparatus. Therefore, each specimen was made into cell suspension of 250cells/0.01ml. After shieving and syringing them using a combination of stainless mesh and nuclepore filter (12μ), specimen were smeared on the slide glass. In field tests using such smear specimens, the false positive rate was 34% in case of the false negative rate taken as 0, though it was difficult to make a judgment about bloody specimens.
Nowadays, mass population screening of uterine carcinoma has been greatly increasing the number of specimens for cytodiagnosis, giving a great burden to cytoscreeners. However, the larger part of the specimens belongs to the normal group.
As shown above, screening by the automatic sie v ing apparatus with 4 basic items as parameters has revealed that nearly half of the normal specimens are accurately excluded as normal. And so, remained half of normal specimens diagnosed as abnormal and other abnormal specimens must be examined by cytoscreeners.
This is the reason why we atte m pted to make the present study.
