The Journal of Medical Investigation
Online ISSN : 1349-6867
Print ISSN : 1343-1420
Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome
Keigo KinoshitaToshikatsu ShinkaYouichi SatoHiroki KurahashiHiroe KowaGang ChenMayumi UmenoKazunori ToidaEmi KiyokageTakuro NakanoSusumu ItoYutaka Nakahori
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2006 Volume 53 Issue 1,2 Pages 117-122


Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence [mHSFYL (Riken cDNA 4933413G11Rik)], which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA- or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis. J. Med. Invest. 53: 117-122, February, 2006

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© 2006 by The University of Tokushima Faculty of Medicine
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