Abstract
The fatty acid content of bovine serum albumin fraction-V (BSA-V) was analysed and the effect of fatty acids bounded with BSA was evaluated. Gas-liquid chromatography was employed to analyse the fatty acids contents and culture experiments were conducted to evaluate the effect of fatty acids on 4-cell mouse and 8-cell rat embryo development. Linoleic acid was identified to be the major fatty acid (54.55%) bound with BSA, followed by oleic(25.8%), stearic (12.27%) and linolenic(7.36%) acids. BSA binding fatty acids had considerable effect on both mouse and rat embryo development in general but they provided only the rat embryo with energy. The combined effect of different kind of fatty acids (chemically defined lipid concentrate) was more effective in both mouse and rat embryo development.
