Abstract
Bovine embryos derived from IVM, IVF, IVC were subjected to analyze of programmed SDS-electrophoresis with silver staining, using Phast system (Pharmacia Co. Ltd.). Embryos in early stage of development with immatured oocytes, matured oocytes, 1 cells (zygotes), 2 cells, 4 cells, 8 cells, morulae and blastocysts were aspirated into 1 μl capillary tube and kept at -60°C until used for electrophoresis. The embryos of each developmental stage were dissolved in saline solution containing SDS and 2-mercaptoethanol, incubate containing for 1 hour at 60°C, and applied to the electrophoresis. One or three embryos were standarized to analyze their protein pattern in each stage. The pattern of protein bands were analyzed with a densitometer (CS-9300-PC, Shimadzu). The band of 59-62 KD in immatured oocytes become high molecular weights eggs developed. The band of 84-91 KD and 101-108 KD were identified in eggs at each stage of the early development except for 101-108 KD in immature oocytes. In addition the bovine embryos during early development had the protein bands that were the same bands recognized as 101-103 KD in granulosa cells, 63-65 KD in FCS and follicular fluid, respectively.
