Abstract
The effect of the prostaglandin E2, F2α and indomethacin on the incorporation of 3H-Methionine in rat blastocyst development was studied by the method of liquid scintillation technique. The eggs of intact pregnant rats when they were on the morula, early blastocyst, blastocyst, and late blastocyst stages were collected. Blastocysts of delayed implantation rats were also collected on the day 6 and 8. The rate of implantation of 3H-Methinine in delayed blastocysts was smaller than in normal blastocysts. Addition of prostaglandin E2 or F2α had accelerated the incorporation of 3H-Methionine in blastocysts at preimplantation and during delayed implantation (P<0.05). But addition of indomethacin inhibited the incorporation of 3H-Methionine in blastocyst and delayed implantation on the day 6 and 8 (P<0.05).
