Journal of Mammalian Ova Research
Online ISSN : 1347-5878
Print ISSN : 1341-7738
ISSN-L : 1341-7738
Original
Comparison of Capacitation and Fertilizing Ability of BALB/c and ICR Mouse Epididymal Spermatozoa: Analysis by In Vitro Fertilization with Cumulus-intact and Zona-free Mouse Eggs
Young-Ho ChoiSeyha SengYutaka Toyoda
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JOURNAL FREE ACCESS

2000 Volume 17 Issue 1 Pages 9-14

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Abstract
The in vitro capacitation and fertilizing ability of epididymal spermatozoa of BALB/c and ICR mice were compared. The capacitation rate of spermatozoa from BALB/c was significantly (P<0.05) lower than that of those from ICR mice when examined by chlortetracycline (CTC) fluorescence assay after 1 and 2 h of preincubation. An in vitro fertilization technique has revealed that the time taken for sperm penetration into intact and zona-free eggs was longer for BALB/c than for ICR spermatozoa (P<0.01). Fertilization rates for spermatozoa from BALB/c mice were significantly (P<0.01) lower than for spermatozoa from ICR regardless of the type of eggs (BALB/c, ICR). These results suggest that the low fertilizing ability of epididymal spermatozoa from BALB/c mice is associated with a lower efficiency of capacitation in vitro.
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© 2000 by Japanese Society of Ova Research
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