Interval between PMSG Priming and hCG Injection in Superovulation of the Mongolian Gerbil

Abstract

To date, a practical method for inducing superovulation in the Mongolian gerbil has not been defined; therefore, in this study, we attempted to develop a flexible superovulation protocol for this species. Superovulation can be induced in the Mongolian gerbils by using PMSG with or without hCG. The injection schedule for PMSG (and hCG) has a high degree of flexibility, but the best protocol for embryo collection for reproductive biology is 20 IU PMSG followed by 20 IU hCG 54 hours later.