Intracytoplasmic Sperm Injection in the Pig: Sperm-based Approaches to Improve Viability of Embryos Generated by ICSI

Abstract

It has been a problem that the rate of development of porcine embryos generated by intracytoplasmic sperm injection (ICSI) is quite low. In this issue, we introduce ideas from recent studies to improve the developmental competence of porcine ICSI embryos: uniqueness in physiology of fertilization by ICSI, and the effects of sperm preparation before ICSI on completion of fertilization and embryonic development following ICSI.